ICMCTF2016 Session D1: Anti-Bacterial Coatings, Surface Functionalization, Surgical Instruments

Tuesday, April 26, 2016 8:00 AM in Room Sunrise

Tuesday Morning

Start	Invited?	Item
8:00 AM		D1-1 Adhesion Inhibitory Effects of Thin Film Metallic Glass on Various Cancer and Platelet Cells Chia-Lin Li (National Taiwan University of Science and Technology, Taiwan, Republic of China); Ming-Jen Chen, Shih-Hsin Chang (MacKay Memorial Hospital Tamsui Campus, Taiwan, Republic of China); Jinn P. Chu (National Taiwan University of Science and Technology, Taiwan, Republic of China) Cancer cell adhesion to surgical instruments is highly undesirable because it may lead to detrimental spreads of cancer cells. Meanwhile, platelet adhesion and aggregation within the intravenous catheter can cause increased chances of thrombus formation and luminal occlusion. In this study, we examined adhesion inhibitory effects of thin film metallic glass (TFMG) coating on various cancer and platelet cells. TFMG is selected as the coating material for its unique properties such as good biocompatibility and antibacterial property due to its random-packing atomic structure, suggesting a great potential for biomedical applications. For comparisons, the adhesions of cancer cells and platelets to Ti coatings are also measured. TFMG coating is shown to minimize the attachment area of various cancer cells by 70-85% in relative to that of uncoated surface. Compared to that of bare surface, TFMG coating is also shown to reduce ~80% and ~70% of attachment area for human and pig platelets, respectively. In this presentation, the morphologies of cancer cell and platelets on both coatings, along with contact angle measurement results, are presented. A possible mechanism for this growth and adhesion inhibitory effect is proposed.
8:20 AM		D1-2 Effects of Oxygen Concentration on the Anti-bacteria Property of AgxO and TaON-Ag JhangHsing Hsieh, ChiaYu Lin, ShuJian Liao (Ming Chi University of Technology, Taiwan, Republic of China); Chuan Li (National Yang Ming University, Taiwan, Republic of China); YiChih Lin (Ming Chi University of Technology, Taiwan, Republic of China) AgxO and TaOxNy-Ag thin films were deposited by reactive co-sputtering with various oxygen flow rates. After deposition and rapid-thermal-annealing at 400 oC for 4 min., the films' structural and mechanical properties were examined. Then, the samples were tested for their antibacterial behaviors against Escherichia coli. It was first found that the dissolution of Ag ion was varied depending on oxygen contents. This happened on both AgxO and TaOxNy-Ag. The Ag ion concentration would reach a maximum value with the increase of oxygen contents, then level off. The antibacterial efficiency of TaOxNy-Ag films against Escherichia coli could be much improved, comparing with that of TaN-Ag films, i.e. the higher oxygen content, the better antibacterial efficiency. The reasons for this were found to be due to smaller metal particles for TaOxNy-Ag, and, more importantly, the existence of silver oxide. This result was proved with ICP-OES by measuring the solubility of metal ions.
8:40 AM	Invited	D1-3 Silver Nano Particle Containing Diamond Like Carbon: An Antimicrobial and Wear Resistant Surface Modification Sascha Buchegger, Caroline Vogel, Eliane Blauth, Melanie Stamp, Anna Joetten, Christoph Westerhausen, Bernd Stritzker, Achim Wixforth (University of Augsburg, Germany) Due to the demographic change, there is an increasing number of people of higher age. Hence, there is also a growing need for total joint replacements, e.g. hip- or knee prosthesis. To lower the number of revision surgeries and to reduce stress for the patients, it is of paramount importance to extend the lifetime of such implants and additionally improve wound healing properties. Our contribution to achieve these aims are hard and wear-resistant diamond-like carbon (DLC) surfaces in combination with various orthopaedic base materials. Additional biofunctionality of the surface modification is achieved by antimicrobial effective silver nanoparticles. We modify our surfaces by two different methods: In the coating method we deposit a polymer film containing silver nanoparticle on the substrate using a sol-gel process and afterwards we transform the polymer layer into DLC by plasma immersion ion implantation. Whereas the modification method consists of the implantation of silver ions in polymer substrates followed by the transformation of the polymer surface into diamond-like carbon by ion irradiation. To prove the successful DLC transformation, we measured both the typical content of sp3-bindings of roughly 35% and the increased nano-hardness up to 14 GPa. Under physiological conditions in vitro, silver ion release was determined with decay-times between two hours and up to six days with an initial silver ion release of 0,61µg/cm2 up to 3,69µg/cm2. To study the adhesion properties of clinically relevant cell lines on such implant surfaces, we present a microfluidic, acoustically driven reaction chamber. Here, a quantitative in vitro investigation of the cell adhesion employing living cells becomes feasible, which will be also presented.