AVS2010 Session PS2+BI-ThA: Plasmas for Medical and Biological Applications

Thursday, October 21, 2010 2:00 PM in Room Galisteo
Thursday Afternoon

Time Period ThA Sessions | Abstract Timeline | Topic PS Sessions | Time Periods | Topics | AVS2010 Schedule

Start Invited? Item
2:00 PM Invited PS2+BI-ThA-1 Activation of Cell under the Atmospheric Pressure Plasma Irradiation
Takamichi Hirata, Chihiro Tsutsui, Akira Mori, Toshiaki Yamamoto, Akira Taguchi (Tokyo City University, Japan)

The researches in the case of “novel plasma” have been widely conducted in the fields of chemistry, solid physics, and nanomaterial science. Such plasma uses a boundary reaction field in a liquid or gaseous-liquid phase based on application of liquid plasma, micro plasma, and atmospheric pressure plasma. In particular, atmospheric pressure plasma is indispensable not only for sterilization, disinfection, decomposition of hazardous materials, and surface modification but also for the cultivation and development of complex new areas which require a diverse perspective, involving biomedical science. From the above-mentioned background, we are conducting basic experiments on direct irradiation of cells using a micro-spot atmospheric pressure plasma source.

The device is a coaxial structure having a tungsten wire (1 mm I.D.) installed inside a glass capillary (plasma generation area: 8 mm I.D.; tip area: 1 mm I.D.), and a grounded tubular electrode wrapped on the outside. The high voltage for the plasma generation is provided by the high voltage power supply. The conditions of plasma generation are as follows: applied voltage: 5-9 kV, frequency: 1-3 kHz, helium (He) gas flow rate: 1 L/min, and plasma irradiation time: 1-300 sec. The experiment was conducted by preparing a culture medium containing mouse fibroblasts (NIH3T3) on a culture dish (made of polypropylene). A culture dish irradiated with plasma was introduced into a CO2-incubator.

According to the dependency of cell numbers against the plasma irradiation time, when only He gas was flowed, the growth of cells was inhibited as the floatation of cells caused by gas agitation inside the culture was promoted. On the other hand, there was no floatation of cells and healthy growth was observed when plasma was generated. Therefore, it appears that the interaction due to ion/radical collisions on the culture surface causes a substantial effect on the proliferation of growth factors such as epidermal growth factor ( EGF), nerve growth factor (NGF), and transforming growth factor ( TGF) that are present in the cells.

2:40 PM PS2+BI-ThA-3 Stability of Highly Functionalised Plasma Polymerised Acrylic Acid Thin Films in Aqueous Environments
Christopher Easton, Adoracion Pegalajar Jurado, Albert Badri, Sally L. McArthur (Swinburne University of Technology, Australia)

Plasma polymerisation provides a convenient one step method for creating a functionalised organic surface on virtually any substrate. This technique has attracted considerable attention in recent years for application within the biomedical field as a substrate for cell culture and as a surface functionalisation for polymer grafting and protein immobilisation [1-4]. Detailed stability studies of these coatings in aqueous solutions have focused on water rather than more biological relevant solutions including phosphate buffered saline (PBS). Critically, the interplay between coating stability and protein and polymer adsorption on the coating behaviours have rarely been examined.

Within this study, highly functionalised acrylic acid thin films have been fabricated via RF plasma polymerisation and the stability of these coatings in aqueous environments examined. The chemical and physical stability of these coatings in water and PBS were investigated using X-ray Photoelectron Spectroscopy (XPS), Atomic Force Microscopy (AFM) and Quartz Crystal Microbalance with Dissipation (QCM-D). The results have shown that the physical behaviour of the coatings changes significantly when they are exposed to water and buffers with differing pH and ionic strength. The significance of these stability observations in an application setting has been explored where the plasma polymerised acrylic acid coating has been used in the assembly of polyelectrolyte layers and biomolecule immobilisation.

References:

[1] K. S. Siow, L. Britcher, S. Kumar, H. J. Griesser, Plasma Process. Polym. 2006, 3, 392.

[2] R. Forch, A. N. Chifen, A. Bousquet, H. L. Khor, M. Jungblut, L. Q. Chu, Z. Zhang, I. Osey-Mensah, E. K. Sinner, W. Knoll, Chem. Vapor Depos. 2007, 13, 280.

[3] H. E. Colley, G. Mishra, A. M. Scutt, S. L. McArthur, Plasma Process. Polym. 2009, 6, 831.

[4] G. J. S. Fowler, G. Mishra, C. D. Easton, S. L. McArthur, Polymer 2009, 50, 5076.

3:00 PM PS2+BI-ThA-4 Scalable Atmospheric DBD Device for Biomedical Processing
Satoshi Kitazaki, Takurou Iwao, Giichiro Uchida, Kazunori Koga, Masaharu Shiratani (Kyushu University, Japan); Nobuya Hayashi (Saga University, Japan)

Nonthermal atmospheric discharge plasmas have been employed for biomedical processing applications, because they offer low temperature processing [1-3]. We have developed a scalable atmospheric dielectric barrier discharge (DBD) device for biomedical processing in a large area. The device consists of 12 electrodes of a stainless rod of 1 mm in outer diameter and 60 mm in length covered with a ceramic tube of 2 mm in outer diameter. In principle, the device size can be extended to a large area by increasing the electrode length as well as the number of electrodes. The electrodes are arranged parallel with each other at a distance of 0.5 mm. The frequency of applied voltage was 10 kHz, and its peak-to-peak voltage was 10 kV. The peak discharge current was about 0.15 A and the duration of each current pulse was about 10 ns. To obtain information about radicals generated in the discharges, UV-Visible emission spectra were measured with a multi-channel spectrometer. Spectral lines of N2 2nd positive band (280-400 nm) were observed in air DBD discharges. We apply the device to process seeds of radish sprouts. We compare germination and growth of seeds with one minute plasma irradiation to those of seeds without irradiation. While the germination periods of these two kinds of seeds are 2 days, being nearly the same with each other, the growth rate of irradiated seeds is 20-50% faster than that without irradiation. These results suggest that the DBD device is useful for such biomedical processing applications.

[1] J. Raiser and M. Zenker, J. Phys. D, 39, 3520 (2006).

[2] M. G. Kong, et al., New J. Phys., 11, 115012 (2009).

[3] A. Helmke, et al., New J. Phys., 11, 115025 (2009).

3:20 PM BREAK
3:40 PM Invited PS2+BI-ThA-6 2010 AVS Medard Welch Award Lecture - Controlling Plasma Sources: Nano to Bio
Natalia Yu. Babaeva, Sang-Heon Song (University of Michigan, Ann Arbor); Juline Shoeb, Mingmei Wang (Iowa State University); Yang Yang (Applied Materials, Inc.); Mark J. Kushner (University of Michigan, Ann Arbor)

The development of technologies for plasma modification of surfaces is in large part based on controlling plasma sources to deliver desired fluxes of radicals and ions to surfaces. Doing so ultimately rests on the ability to control the energy and velocity distributions of charged and neutral particles. Controlling electron energy distributions, f(e), ultimately specifies the production rates of radicals and ions. Controlling the velocity distributions, f(v), of ions and neutrals ultimately specifies the activation energy delivered to surfaces. There has been an evolution of techniques to control f(e) and f(v) utilizing type of excitation (e.g., ICP vs CCP), frequency, pulse power and, most recently, multiphase plasmas These techniques are being challenged to provide the specificity required for nano-scale processing, particularly given the synergistic and presently uncontrolled relationship between fluxes into and returning from surfaces. Control of f(e) and f(v) becomes even more challenging in biological applications of plasmas and plasma medicine, typically performed at atmospheric pressure, where timescales for plasma formation are shorter than conventional control techniques can address. In this talk, techniques to control f(e) and f(v) in plasma sources in the context of plasma modification of biological and nano-scale surfaces will be discussed. Examples of control techniques will be taken from using pulsed, multi-frequency and multi-phase plasmas. Applications will be discussed from nano-scale cleaning and sealing of porous dielectrics; and dielectric barrier discharge treatment of wounded skin. Challenges facing researchers in developing plasma sources having the ability to control f(e) and f(v) will be discussed.

* Work supported by the Department of Energy Office of Fusion Energy Sciences, Semiconductor Research Corp., Applied Materials, Tokyo Electron., Agilent, Inc.
4:40 PM PS2+BI-ThA-9 Correlation of Properties of Polymeric Organic Layers with Plasma Parameters
Stefan Umrath, Florian Schamberger, Gerhard Franz (Hochschule Muenchen, Germany)

For exact deposition of thin films out of the vaporous phase (cvd), an entire knowledge of the process parameters such as flows, pressure and gaseous temperature is required. In the case of pecvd, this means the extension on influencing plasma variables like plasma density and electron temperature, in particular in large reactors for production purposes to meet the demands for flat layer qualities (growth and composition) over the whole reactor volume.

In an almost cubical reactor 80 l in volume, the microwave power is coupled into the volume via a quartz window which exhibits approximately 1/10 of the sidewall area. The spatial compilation of these plasma quantities along with plasma potential has been accomplished with a bendable Lang­muir probe. To isolate the tungsten wire against its grounded housing tube, it was coated with poly­parylene. After having compared this construction with our Lang­muir probe which has been now in use for more than a decade, we have taken data of the whole reactor with argon and with mixtures of monomers of parylene and argon or oxygen in a pressure range between 10 mTorr and 150 mTorr (1 1/2 Pa to 20 Pa) applying a new evaluation procedure [1]. Over the covered range, the plasma density remains in the dielectric regime (plasma degree less than 100 ppm).

Compared to discharges through pure argon, the plasma parameters exhibit opposing be­havior: at same discharge pressure and power input, the plasma density is lower, whereas the electron temperature goes up. The layers are highly transparent with a slightly yellow color. Ftir measurements reveal that the ring structure still remains intact. Adding oxygen to the ambient to the monomeric vapor leads to hydro­philic surfaces which is caused by the formation of CO bonds and OH bonds. The creation of these features is confined by power input. If it is raised beyond 4 W/l, the reaction mechanism drastically changes from surface polymerization to volume poly­merization lead­ing to thick, low-density films which can be easily be scratched away. This change has been traced by plasma diagnostics and mass spectrometry. At a threshold density of about 1 x 1010cm3 (plasma degree about 1000 ppm), all peaks beyond 44 (CO2) vanish. In the resulting mass spectrum, no CH vibrations beyond 3000 cm-1 can be detected indicating the complete destruction of the aromatic system.

[1] Peter Scheubert: Modelling and Diagnostics of Low Pressure Plasma Discharges, PhD thesis, Bochum, 2002

Time Period ThA Sessions | Abstract Timeline | Topic PS Sessions | Time Periods | Topics | AVS2010 Schedule